{"id":18549,"date":"2020-01-26T09:13:55","date_gmt":"2020-01-26T05:43:55","guid":{"rendered":"https:\/\/digibonyan.com\/?post_type=product&p=18549"},"modified":"2022-01-12T13:22:14","modified_gmt":"2022-01-12T09:52:14","slug":"taq-dna-polymerase%d9%82%d8%af%d8%b1%d8%aa-%da%a9%d8%a7%d8%aa%d8%a7%d9%84%db%8c%d8%b2-dna-%d8%af%d8%b1-%d8%ac%d9%87%d8%aa-5%e2%86%923","status":"publish","type":"product","link":"https:\/\/digibonyan.com\/product\/taq-dna-polymerase%d9%82%d8%af%d8%b1%d8%aa-%da%a9%d8%a7%d8%aa%d8%a7%d9%84%db%8c%d8%b2-dna-%d8%af%d8%b1-%d8%ac%d9%87%d8%aa-5%e2%86%923\/","title":{"rendered":"Taq DNA Polymerase(\u0642\u062f\u0631\u062a \u06a9\u0627\u062a\u0627\u0644\u06cc\u0632 DNA \u062f\u0631 \u062c\u0647\u062a 5\u2019\u21923\u2019)"},"content":{"rendered":"

\u0645\u0639\u0631\u0641\u06cc \u0645\u062d\u0635\u0648\u0644<\/h2>\n

Taq DNA Polymerase \u06cc\u06a9 \u0622\u0646\u0632\u06cc\u0645 \u0628\u0633\u06cc\u0627\u0631 \u0628\u0627 \u062b\u0628\u0627\u062a \u062f\u0631 \u062f\u0631\u062c\u0647 \u062d\u0631\u0631\u0627\u062a \u0628\u0627\u0644\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f.
\nTaq DNA Polymerase \u062f\u0627\u0631\u0627\u06cc \u0642\u0627\u0628\u0644\u06cc\u062a \u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f\u0646 \u0622\u062f\u0646\u06cc\u0646 \u0647\u0627\u06cc \u0627\u0636\u0627\u0641\u06cc \u0631\u0627 \u062f\u0631 \u0627\u0646\u062a\u0647\u0627\u06cc 3\u2019 \u0645\u062d\u0635\u0648\u0644 PCR \u0645\u06cc \u0628\u0627\u0634\u062f.
\n\u0627\u06cc\u0646 \u0622\u0646\u0632\u06cc\u0645 \u0646\u0648\u062a\u0631\u06a9\u06cc\u0628 \u0628\u0631\u0627\u06cc PCR \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u0622\u0645\u067e\u0644\u06cc\u06a9\u0648\u0646\u0647\u0627\u06cc 5 \u06a9\u06cc\u0644\u0648\u0628\u0627\u06cc\u062a \u06cc\u0627 \u06a9\u0648\u062a\u0627\u0647 \u062a\u0631 \u0627\u06cc\u062f\u0647 \u0622\u0644 \u0627\u0633\u062a.
\n\u0627\u06cc\u0646 \u0622\u0646\u0632\u06cc\u0645 \u0642\u062f\u0631\u062a \u06a9\u0627\u062a\u0627\u0644\u06cc\u0632 DNA \u062f\u0631 \u062c\u0647\u062a 5\u2019\u21923\u2019 \u0631\u0627 \u062f\u0627\u0631\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f .
\n\u0645\u0642\u0627\u062f\u06cc\u0631 \u0628\u0647\u06cc\u0646\u0647 \u0627\u0632 DNA \u0627\u0644\u06af\u0648 \u062f\u0631 \u062d\u062c\u0645 \u0648\u0627\u06a9\u0646\u0634 25 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0631\u0627\u06cc DNA \u067e\u0644\u0627\u0633\u0645\u06cc\u062f \u0648 \u0641\u0627\u0698 0.01-1 ng \u0648 \u0628\u0631\u0627\u06cc DNA \u0698\u0646\u0648\u0645\u06cc 0.1-1 \u03bcg \u0627\u0633\u062a.<\/p>\n

 <\/p>\n

\u0648\u06cc\u0698\u06af\u06cc \u0645\u062d\u0635\u0648\u0644<\/h2>\n

\u062f\u0627\u0645\u0646\u0647 \u063a\u0644\u0638\u062a \u062a\u0648\u0635\u06cc\u0647 \u0634\u062f\u0647 \u0627\u0632 Mg2+ 1-4 mM \u0627\u0633\u062a.
\n\u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u062a\u0648\u0635\u06cc\u0647 \u0634\u062f\u0647 \u0627\u0632 \u0647\u0631 dNTP 0.2 mM \u0627\u0633\u062a.
\n\u062f\u0627\u0645\u0646\u0647 \u063a\u0644\u0638\u062a \u062a\u0648\u0635\u06cc\u0647 \u0634\u062f\u0647 \u0627\u0632 \u0622\u063a\u0627\u0632\u06af\u0631\u0647\u0627\u06cc0.1-1 \u03bcM \u0627\u0633\u062a.
\n\u0628\u0631\u0627\u06cc \u0627\u0644\u06af\u0648\u0647\u0627\u06cc \u063a\u0646\u06cc \u0627\u0632 GC \u062f\u0646\u0627\u062a\u0648\u0631\u0627\u0633\u06cc\u0648\u0646 \u0627\u0648\u0644\u06cc\u0647 \u0628\u0627\u06cc\u062f \u062a\u0627 10 \u062f\u0642\u06cc\u0642\u0647 \u0637\u0648\u0644\u0627\u0646\u06cc \u0634\u0648\u062f.
\n\u067e\u06a9\u06cc\u062c \u0627\u06cc\u0646 \u0645\u062d\u0635\u0648\u0644 \u062f\u0627\u0631\u0627\u06cc \u0628\u0627\u0641\u063110X PCR \u0648 50 mM MgCl2 \u0627\u0633\u062a.
\n\u0645\u0642\u062f\u0627\u0631 \u0622\u0646\u0632\u06cc\u0645 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u062a\u06cc\u0648\u067e 100U 1U\/ \u00b5l \u0645\u0639\u0627\u062f\u0644 20 \u00b5l
\n\u0645\u0642\u062f\u0627\u0631 \u0622\u0646\u0632\u06cc\u0645 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u062a\u06cc\u0648\u067e 500U 1U\/ \u00b5l \u0645\u0639\u0627\u062f\u0644 100 \u00b5l
\n\u0645\u0642\u062f\u0627\u0631 \u0622\u0646\u0632\u06cc\u0645 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u062a\u06cc\u0648\u067e 1000U 1U\/ \u00b5l \u0645\u0639\u0627\u062f\u0644 200 \u00b5l<\/p>\n","protected":false},"excerpt":{"rendered":"

Taq DNA Polymerase \u06cc\u06a9 \u0622\u0646\u0632\u06cc\u0645 \u0628\u0633\u06cc\u0627\u0631 \u0628\u0627 \u062b\u0628\u0627\u062a \u062f\u0631 \u062f\u0631\u062c\u0647 \u062d\u0631\u0631\u0627\u062a \u0628\u0627\u0644\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f.
\nTaq DNA Polymerase \u062f\u0627\u0631\u0627\u06cc \u0642\u0627\u0628\u0644\u06cc\u062a \u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f\u0646 \u0622\u062f\u0646\u06cc\u0646 \u0647\u0627\u06cc \u0627\u0636\u0627\u0641\u06cc \u0631\u0627 \u062f\u0631 \u0627\u0646\u062a\u0647\u0627\u06cc 3\u2019 \u0645\u062d\u0635\u0648\u0644 PCR \u0645\u06cc \u0628\u0627\u0634\u062f.
\n\u0627\u06cc\u0646 \u0622\u0646\u0632\u06cc\u0645 \u0646\u0648\u062a\u0631\u06a9\u06cc\u0628 \u0628\u0631\u0627\u06cc PCR \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u0622\u0645\u067e\u0644\u06cc\u06a9\u0648\u0646\u0647\u0627\u06cc 5 \u06a9\u06cc\u0644\u0648\u0628\u0627\u06cc\u062a \u06cc\u0627 \u06a9\u0648\u062a\u0627\u0647 \u062a\u0631 \u0627\u06cc\u062f\u0647 \u0622\u0644 \u0627\u0633\u062a.<\/p>\n","protected":false},"featured_media":18548,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_cat":[268],"product_tag":[1381,1380,1382,440],"class_list":{"0":"post-18549","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-268","7":"product_tag-dna-","8":"product_tag-taq-dna-polymerase","9":"product_tag--pcr","10":"product_tag-440","11":"gallery-content-unit","13":"first","14":"instock","15":"shipping-taxable","16":"purchasable","17":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/product\/18549"}],"collection":[{"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/comments?post=18549"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/media\/18548"}],"wp:attachment":[{"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/media?parent=18549"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/product_cat?post=18549"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/digibonyan.com\/wp-json\/wp\/v2\/product_tag?post=18549"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}